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We have developed a method for the isolation of brush border membrane fragments from proximal renal tubules using percoll purified glomeruli. The procedure consists in the incubation of renal cortex homogenates with percoll and centrifugation at 100,000 x g for 1 hr. This method provides highly purified brush border vesicles. A two-step incubation procedure, i.e. preincubation of the percoll supernatant with MgCl2, was used to inhibit glomerular basement membrane, followed by the incubation of the non-bound brush border membrane with ATP, isocaproic acid and carbonate buffer, for measuring the ion transport properties of the isolated brush border membrane. The ATP-dependent transport of Na+ and H+ were similar to those found in brush border membrane vesicles isolated by the more classical procedure and the initial rate of Na+ and H+ uptake is characterized by a high affinity and a low capacity.Amar Singh’s star is indeed on the rise and he had finally proved that he is ready to play a role in a film. We have already seen ‘Son of Sardar’ receiving high praises from all the corners. This is a rare moment for an actor that is not just being appreciated for his on-screen skills, but his real life persona also. Coming to this particular film, it has been known for a long time that Amar has already made his mark as a character actor, but this is one performance that will surely be remembered for a long time.
Amar is a sadhu and he lives in the jungles along with his brother and their two disciples who help him in his mission. A gangster Amba (Emraan Hashmi) on the other hand wants to do a deal with Amar and wants him to get his wife back. Amba promises Amar that he will send his wife out of jail before the cut in the deal. The sadhu doesn’t believe this, but he accepts.
The movie is one that is not laced with any scenes. The story will take some time to unfold, which is really surprising as the supporting characters in the movie play a huge part in shaping the story. In the movie, Amba goes to receive help from the people who had helped him in the past. There is a special bond between Amar and them, but he always wanted to prove them
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